HTR2C Antikörper (AA 439-460)

Produktnummer ABIN617931

Produktdetails anti-HTR2C Antikörper

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Bindungsspezifität: AA 439-460

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HTR2C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Produktmerkmale: The 5-HT 2C Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 439-460 of the rat 5-HT2C receptor coupled to KLH and bovine thyroglobulin. The 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.

Aufreinigung: Affinity Purified

Immunogen: Rat 5-HT2C receptor (439-460)

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

Referenzen für anti-HTR2C Antikörper (ABIN617931)

Zhang, Li, Xin, Lao, Ren, Berman, Tan, Zhang: "Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model." in: Neurochemical research, Vol. 36, Issue 10, pp. 1785-92, (2011) (PubMed).

Coulon, Kanyshkova, Broicher, Munsch, Wettschureck, Seidenbecher, Meuth, Offermanns, Pape, Budde: "Activity Modes in Thalamocortical Relay Neurons are Modulated by G(q)/G(11) Family G-proteins - Serotonergic and Glutamatergic Signaling." in: Frontiers in cellular neuroscience, Vol. 4, pp. 132, (2011) (PubMed).

Yang, Mahaffey, Bérubé, Maddatu, Cox, Frankel: "Complex seizure disorder caused by Brunol4 deficiency in mice." in: PLoS genetics, Vol. 3, Issue 7, pp. e124, (2007) (PubMed).

Holmes: "5-Hydroxytryptamine2C receptors on pudendal motoneurons innervating the external anal sphincter." in: Brain research, Vol. 1057, Issue 1-2, pp. 65-71, (2005) (PubMed).

Details zu HTR2C

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Andere Bezeichnung: Serotonin Receptor 2C (HTR2C) (HTR2C Produkte)

Hintergrund: Other Names:
5-hydroxytryptamine receptor 2C,5-HT2C, 5HT-1C, 5-HTR2C,5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled

Gen-ID: 25187

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour