Serotonin Receptor 5A Antikörper (AA 17-34)

Produktnummer ABIN617921

Der Kaninchen Polyklonal Anti-Serotonin Receptor 5A-Antikörper wurde für WB, IF, IHC, ICC und IEM validiert. Er ist geeignet, Serotonin Receptor 5A in Proben von Ratte und Clams (Mytilus) zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Serotonin Receptor 5A Antikörper (AA 17-34) (ABIN617921)

Target: Serotonin Receptor 5A (HTR5A)

Reaktivität: Ratte, Clams (Mytilus)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Produktdetails anti-Serotonin Receptor 5A Antikörper

Bindungsspezifität: AA 17-34

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: The 5-HT 5A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde. The 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS/0.3 % Triton X-100 - Cy3 and 1/200 - 1/400 in PBS/0.3 % Triton X-100 - Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

Aufreinigung: Affinity Purified

Immunogen: Rat 5-HT5A receptor (17-34)

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

Referenzen für anti-Serotonin Receptor 5A Antikörper (ABIN617921)

Kreiling, Stephens, Reinisch: "A mixture of environmental contaminants increases cAMP-dependent protein kinase in Spisula embryos." in: Environmental toxicology and pharmacology, Vol. 19, Issue 1, pp. 9-18, (2011) (PubMed).

Doly, Fischer, Brisorgueil, Vergé, Conrath: "5-HT5A receptor localization in the rat spinal cord suggests a role in nociception and control of pelvic floor musculature." in: The Journal of comparative neurology, Vol. 476, Issue 4, pp. 316-29, (2004) (PubMed).

Details zu Serotonin Receptor 5A

Target: Serotonin Receptor 5A (HTR5A)

Hintergrund: Other Names:
5-hydroxytryptamine receptor 5A, 5-HT5A, REC17,5ht5a,MR22,5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled

Gen-ID: 25689

Pathways: JAK-STAT Signalweg