CD55 Antikörper (CF®568)

Produktnummer ABIN6177466

Der Maus Monoklonal Anti-CD55-Antikörper wurde für FACS, IF und IHC (fro) validiert. Er ist geeignet, CD55 in Proben von Human zu detektieren.

Kurzübersicht für CD55 Antikörper (CF®568) (ABIN6177466)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD55 Antikörper ist konjugiert mit CF®568

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 143-30

Produktdetails anti-CD55 Antikörper (CF®568)

Verwendungszweck: Mouse Monoclonal anti-CD55 (DAF) (143-30), CF568 Conjugate

Produktmerkmale: This antibody recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: PHA stimulated human PBL

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes. Human lymph nodes and tonsils.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Andere Bezeichnung: CD55 (DAF)

Molekulargewicht: 70 kDa

Gen-ID: 1604, 126517

UniProt: P08174

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity