CD3 epsilon Antikörper

Produktnummer ABIN6176615

Der Maus Monoklonal anti-CD3 epsilon Antikörper (Klon CRIS-7) (ABIN6176615) detektiert spezifisch CD3 epsilon in FACS, IF, Act und ProA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für CD3 epsilon Antikörper (ABIN6176615)

Target: CD3 epsilon (CD3E)

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Activation (Act), Proliferation Assay (ProA)

Klon: CRIS-7

Produktdetails anti-CD3 epsilon Antikörper

Verwendungszweck: Mouse Monoclonal anti-CD3e (CRIS-7), Purified, BSA-free

Produktmerkmale: This antibody recognizes the epsilon-chain of CD3 (Workshop V, Code: CD03.09), which consists of five different polypeptide chains (designated as γ, δ, ε, ζ, and η) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Stimulated human leukocytes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Induce T cell activation and proliferation
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat cells. Tonsil and lymph node

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3e

Molekulargewicht: 20 kDa

Gen-ID: 916, 3003

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway