BCL2L1 Antikörper (AA 3-14) (CF®488A)

Produktnummer ABIN6175460

Dieses Anti-BCL2L1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von BCL2L1 in WB, IF, FACS und IHC (f). Geeignet für Human, Maus, Ratte und Schwein.

Kurzübersicht für BCL2L1 Antikörper (AA 3-14) (CF®488A) (ABIN6175460)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCL2L1 Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: BX006 2H12

Produktdetails anti-BCL2L1 Antikörper (CF®488A)

Bindungsspezifität: AA 3-14

Verwendungszweck: Mouse Monoclonal anti-bcl-x (BX006 + 2H12), CF488A Conjugate

Produktmerkmale: This antibody recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: bcl-x

Hintergrund: Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)

Molekulargewicht: 27 kDa

Gen-ID: 598, 516966

UniProt: Q07817

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling