beta-2 Microglobulin Antikörper (CF®543)

Produktnummer ABIN6175216

Der Maus Monoklonal Anti-beta-2 Microglobulin-Antikörper wurde für FACS, IF und IHC (fro) validiert. Er ist geeignet, beta-2 Microglobulin in Proben von Human und Non-Human Primate zu detektieren.

Kurzübersicht für beta-2 Microglobulin Antikörper (CF®543) (ABIN6175216)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta-2 Microglobulin Antikörper ist konjugiert mit CF®543

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: BBM-1

Produktdetails anti-beta-2 Microglobulin Antikörper (CF®543)

Verwendungszweck: Mouse Monoclonal anti-Beta-2 Microglobulin (B2M) (BBM.1), CF543 Conjugate

Produktmerkmale: This antibody recognizes a protein of 12 kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an α heavy chain that contains three subdomains (α1, α2, α3) and a non-covalent associating light chain, known as β-2-Microglobulin. β-2-Microglobulin associates with the α3 subdomain of the α heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The α1 and α2 domains of the α heavy chain form the peptide antigen-binding cleft. Mutations in the β-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: MOLT-4 human T cell line

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung: Beta-2 Microglobulin (B2M)

Hintergrund: B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p

Molekulargewicht: 12 kDa

Gen-ID: 567, 534255

UniProt: P61769

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process