EPO Antikörper (AA 28-162) (CF®488A)

Produktnummer ABIN6174282

Dieses Maus Monoklonal-Antikörper erkennt spezifisch EPO in WB, IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EPO Antikörper (AA 28-162) (CF®488A) (ABIN6174282)

Target: EPO (Erythropoietin (EPO))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPO Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: EPO-1368

Produktdetails anti-EPO Antikörper (CF®488A)

Bindungsspezifität: AA 28-162

Verwendungszweck: Mouse Monoclonal anti-Erythropoietin (EPO/1368), CF488A Conjugate

Produktmerkmale: This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human EPO protein (aa28-162) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 4-8 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HepG2 Cells. Heart or Kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu EPO

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: Erythropoietin

Substanzklasse: Hormone

Hintergrund: EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2

Molekulargewicht: 37 kDa

Gen-ID: 2056, 2303

UniProt: P01588

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity