Cytochrome C Antikörper (CF®640R)

Produktnummer ABIN6170388

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytochrome C in WB, FACS, IF und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster und Frosch.

Kurzübersicht für Cytochrome C Antikörper (CF®640R) (ABIN6170388)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist konjugiert mit CF®640R

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CTC05

Produktdetails anti-Cytochrome C Antikörper (CF®640R)

Verwendungszweck: Mouse Monoclonal anti-Cytochrome-C (CTC05), CF640R Conjugate

Produktmerkmale: Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant cytochrome c protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: Cytochrome-C

Hintergrund: CYC, CYCS, HCS, THC4

Molekulargewicht: 15 kDa

Gen-ID: 54205, 437060

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity