Rekombinanter Cytokeratin 7 Antikörper
Kurzübersicht für Rekombinanter Cytokeratin 7 Antikörper (ABIN6170345)
Target
Alle Cytokeratin 7 (KRT7) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Recombinant Rabbit Monoclonal anti-Cytokeratin 7 (KRT7/1499R), Purified, BSA-free
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Produktmerkmale
- This antibody recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Aufreinigung
- Purified
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Immunogen
- Recombinant full-length human KRT7 protein
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Isotyp
- IgG kappa
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Applikationshinweise
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Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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Kommentare
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HeLa cells. Carcinoma of ovary, lung, cervix, or breast
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- PBS (no BSA, no azide)
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Konservierungsmittel
- Azide free
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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Andere Bezeichnung
- Cytokeratin 7
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Hintergrund
- CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
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Molekulargewicht
- 55 kDa
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Gen-ID
- 3855, 411501
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UniProt
- P08729
Target
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