Cytokeratin 7 Antikörper (CF®405S)

Produktnummer ABIN6170118

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 7 in IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für Cytokeratin 7 Antikörper (CF®405S) (ABIN6170118)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 7 Antikörper ist konjugiert mit CF®405S

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: KRT7-760 OV-TL12-30

Produktdetails anti-Cytokeratin 7 Antikörper (CF®405S)

Verwendungszweck: Mouse Monoclonal anti-Cytokeratin 7 (KRT7/760 + OV-TL12/30), CF405S Conjugate

Produktmerkmale: This antibody recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human KRT7 protein (KRT7/760), OTN 11, ovarian carcinoma cell line (OV-TL12/30)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa cells. Carcinoma of ovary, lung, cervix, or breast

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Andere Bezeichnung: Cytokeratin 7

Hintergrund: CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Molekulargewicht: 55 kDa

Gen-ID: 3855, 411501

UniProt: P08729