N-Cadherin Antikörper (Cytoplasmic Domain) (CF®568)

Produktnummer ABIN6169562

Dieses Anti-N-Cadherin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von N-Cadherin in WB, IF und FACS. Geeignet für Human und Maus.

Kurzübersicht für N-Cadherin Antikörper (Cytoplasmic Domain) (CF®568) (ABIN6169562)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser N-Cadherin Antikörper ist konjugiert mit CF®568

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 13A9

Produktdetails anti-N-Cadherin Antikörper (CF®568)

Bindungsspezifität: Cytoplasmic Domain

Verwendungszweck: Mouse Monoclonal anti-N-Cadherin / Cadherin-2 / CD325 (13A9), CF568 Conjugate

Produktmerkmale: This antibody recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human N-cadherin cytoplasmic domain (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: N-Cadherin / Cadherin-2 / CD325

Hintergrund: Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD

Molekulargewicht: 130-140 kDa

Gen-ID: 1000, 464829

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane