ICAM-3/CD50 Antikörper (CF®568)

Produktnummer ABIN6169448

Dieses Anti-ICAM-3/CD50-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ICAM-3/CD50 in FACS, IF und IHC (f). Geeignet für Human.

Kurzübersicht für ICAM-3/CD50 Antikörper (CF®568) (ABIN6169448)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM-3/CD50 Antikörper ist konjugiert mit CF®568

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 186-2G9

Produktdetails anti-ICAM-3/CD50 Antikörper (CF®568)

Verwendungszweck: Mouse Monoclonal anti-CD50 (ICAM-3) (186-2G9), CF568 Conjugate

Produktmerkmale: This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Blocks binding of CD11a LFA-1 to CD50 ICAM-3
• Optimal dilution for a specific application should be determined by user

Kommentare:

HL-60 or THP-1 cells. Lymph node and tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Andere Bezeichnung: CD50 (ICAM-3)

Hintergrund: ICAMR, Intercellular adhesion molecule 3 (ICAM3)

Molekulargewicht: 110-160 kDa

Gen-ID: 3385, 354563

UniProt: P32942