alpha Fetoprotein Antikörper (CF®647)

Produktnummer ABIN6168492

Dieses Maus Monoklonal-Antikörper erkennt spezifisch alpha Fetoprotein in IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Schwein, Hund und Affe.

Kurzübersicht für alpha Fetoprotein Antikörper (CF®647) (ABIN6168492)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human, Schwein, Hund, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist konjugiert mit CF®647

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: C3

Produktdetails anti-alpha Fetoprotein Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-AFP (Alpha-Fetoprotein) (C3), CF647 Conjugate

Produktmerkmale: This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Does not react with cow, dog, mouse, or rat
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: AFP (Alpha-Fetoprotein)

Hintergrund: Alpha fetoglobulin, FETA, HPAFP

Molekulargewicht: 70 kDa

Gen-ID: 174, 518808

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process