NUMA1 Antikörper

Produktnummer ABIN6165954

Produktdetails anti-NUMA1 Antikörper

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NUMA1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-NuMA (A73-B/D12), Purified, BSA-free

Produktmerkmale: This antibody recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Colon carcinoma 174T cells

Klon: A73-B-D12

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Exponentially growing any cultured human cells. Tonsil or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Andere Bezeichnung: NuMA (NUMA1 Produkte)

Synonyme: NUMA antikoerper, 6720401E04Rik antikoerper, AA764025 antikoerper, AL022610 antikoerper, AU014979 antikoerper, NUMA1 antikoerper, NuMA antikoerper, SP-H antikoerper, numa antikoerper, numa1 antikoerper, nuclear mitotic apparatus protein 1 antikoerper, nuclear mitotic apparatus protein 1 S homeolog antikoerper, NUMA1 antikoerper, Numa1 antikoerper, numa1 antikoerper, numa1.S antikoerper

Hintergrund: Nuclear Mitotic Apparatus Protein 1, NuMA protein, NUMA1, SP-H antigen, Structural nuclear protein,

Molekulargewicht: 228 kDa

Gen-ID: 4926, 325978

UniProt: Q14980

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, M Phase