Involucrin Antikörper

Produktnummer ABIN6164850

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Involucrin in IF, FACS, ISt und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Hund, Schwein, Gorilla, Affe und Owl.

Kurzübersicht für Involucrin Antikörper (ABIN6164850)

Target: Involucrin (IVL)

Reaktivität: Human, Hund, Schwein, Gorilla, Affe, Owl

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Involucrin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: SY5

Produktdetails anti-Involucrin Antikörper

Verwendungszweck: Mouse Monoclonal anti-Involucrin (SY5), Purified, with BSA

Produktmerkmale: This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Purified involucrin from human keratinocytes

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37C, enzyme digestion is better than citrate buffer based epitope unmasking
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence
• Does not react with mouse, others not known
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Involucrin

Target: Involucrin (IVL)

Andere Bezeichnung: Involucrin

Hintergrund: IVL

Molekulargewicht: 66-170 kDa

Gen-ID: 3713, 516439

UniProt: P07476