Rekombinanter Chromogranin A Antikörper (CF®405S)

Produktnummer ABIN6160940

Der Kaninchen Monoklonal Anti-Chromogranin A-Antikörper wurde für IF, IHC, FACS und WB validiert. Er ist geeignet, Chromogranin A in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Chromogranin A Antikörper (CF®405S) (ABIN6160940)

Target: Chromogranin A (CHGA)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Chromogranin A Antikörper ist konjugiert mit CF®405S

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Klon: CHGA-1815R

Produktdetails anti-Chromogranin A Antikörper (CF®405S)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-Chromogranin A / CHGA (CHGA/1815R), CF405S Conjugate

Produktmerkmale: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CHGA protein

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Chromogranin A

Target: Chromogranin A (CHGA)

Andere Bezeichnung: Chromogranin A / CHGA

Hintergrund: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Pituitary Secretory Protein I, SP-I, Vasostatin I or II

Molekulargewicht: 68-75 kDa

Gen-ID: 1113, 150793

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling