CD53 Antikörper (CF®594)

Produktnummer ABIN6159823

Der Maus Monoklonal Anti-CD53-Antikörper wurde für FACS und IF validiert. Er ist geeignet, CD53 in Proben von Human zu detektieren.

Kurzübersicht für CD53 Antikörper (CF®594) (ABIN6159823)

Target: CD53

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD53 Antikörper ist konjugiert mit CF®594

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 161-2

Produktdetails anti-CD53 Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-CD53 (161-2), CF594 Conjugate

Produktmerkmale: This antibody recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI, Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb This antibody recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Does not react with baboon or horse, others not tested

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53

Hintergrund: CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25

Molekulargewicht: 33-55 kDa

Gen-ID: 963, 443057

UniProt: P19397