UBE2F Antikörper (N-Term)

Produktnummer ABIN615974

Der Ziege Polyklonal anti-UBE2F Antikörper wird verwendet zum Nachweis von UBE2F in Proben von Human, Maus, Ratte, Rind (Kuh), Hund und Schwein. Er wurde validiert für WB und EIA.

Kurzübersicht für UBE2F Antikörper (N-Term) (ABIN615974)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser UBE2F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-UBE2F Antikörper

Bindungsspezifität: N-Term

Sequenz: SKLKRDDGLK GSRT-C

Spezifität: Recogfnizes NEDD8 conjugating enzyme (N Terminus).

Kreuzreaktivität (Details): Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Andere Bezeichnung: NCE2

Hintergrund: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F

Gen-ID: 140739

NCBI Accession: NP_542409

UniProt: Q969M7