HSPG Antikörper (CF®488A)

Produktnummer ABIN6159593

Dieses Anti-HSPG-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von HSPG in IHC (p), IF, FACS und IHC (f). Geeignet für Human, Rind (Kuh), Maus, Fisch, Affe und Schwein.

Kurzübersicht für HSPG Antikörper (CF®488A) (ABIN6159593)

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Reaktivität: Human, Rind (Kuh), Maus, Fisch, Affe, Schwein

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSPG Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: A7L6

Produktdetails anti-HSPG Antikörper (CF®488A)

Verwendungszweck: Rat Monoclonal anti-Heparan Sulfate Proteoglycan (A7L6), CF488A Conjugate

Produktmerkmale: This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Murine EHS laminin preparation

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Breast carcinomas, squamous cell carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu HSPG

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Andere Bezeichnung: Heparan Sulfate Proteoglycan

Hintergrund: Heparan sulfate proteoglycan of basement membrane, HSPG, Hspg2, LG3 peptide, Perlecan, PLC, SJS1

Molekulargewicht: >400 kDa

Gen-ID: 3339, 562227

UniProt: P98160