Androgen Receptor Antikörper (AA 299-315) (CF®640R)

Produktnummer ABIN6156079

Der Maus Monoklonal Anti-Androgen Receptor-Antikörper wurde für IF und IHC (f) validiert. Er ist geeignet, Androgen Receptor in Proben von Human zu detektieren.

Kurzübersicht für Androgen Receptor Antikörper (AA 299-315) (CF®640R) (ABIN6156079)

Target: Androgen Receptor (AR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Androgen Receptor Antikörper ist konjugiert mit CF®640R

Applikation: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: AR441 DHTR-882

Produktdetails anti-Androgen Receptor Antikörper (CF®640R)

Bindungsspezifität: AA 299-315

Verwendungszweck: Mouse Monoclonal anti-Androgen Receptor (AR441 + DHTR/882), CF640R Conjugate

Produktmerkmale: This antibody recognizes a protein of 110 kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin.This MAb is superb for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide, aa 299-315, (STEDTAEYSPFKGGYTK) of human AR (AR441), Recombinant human DHTR protein (DHTR/882)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Does not react with mouse, others not known
• Optimal dilution for a specific application should be determined by user

Kommentare:

LNCap cells or Prostate carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Androgen Receptor

Target: Androgen Receptor (AR)

Andere Bezeichnung: Androgen Receptor

Molekulargewicht: ~110 kDa

Gen-ID: 367, 496240

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding