ADRP Antikörper (AA 249-376) (CF®594)

Produktnummer ABIN6155757

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ADRP in IHC, ELISA, IF und Coat. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ADRP Antikörper (AA 249-376) (CF®594) (ABIN6155757)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ADRP Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat)

Klon: ADFP-1366

Produktdetails anti-ADRP Antikörper (CF®594)

Bindungsspezifität: AA 249-376

Verwendungszweck: Mouse Monoclonal anti-Adipophilin / Perilipin-2 (ADFP/1366), CF594 Conjugate

Produktmerkmale: This antibody recognizes a protein of 48 kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Adipophilin protein fragment (aa249-376) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Kommentare:

HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Andere Bezeichnung: Adipophilin / Perilipin-2

Hintergrund: Adipophilin, ADFP, Adipose differentiation-related protein (ADRP), Perilipin-2 (PLIN2)

Molekulargewicht: 48 kDa

Gen-ID: 123, 3416

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism