Aurora Kinase B Antikörper (AA 89-251) (CF®647)

Produktnummer ABIN6155551

Der Maus Monoklonal Anti-Aurora Kinase B-Antikörper wurde für WB, IF und FACS validiert. Er ist geeignet, Aurora Kinase B in Proben von Human zu detektieren.

Kurzübersicht für Aurora Kinase B Antikörper (AA 89-251) (CF®647) (ABIN6155551)

Target: Aurora Kinase B (AURKB)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: AURKB-1521

Produktdetails anti-Aurora Kinase B Antikörper (CF®647)

Bindungsspezifität: AA 89-251

Verwendungszweck: Mouse Monoclonal anti-Aurora-B (AURKB/1521), CF647 Conjugate

Produktmerkmale: This antibody recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Aurora B protein fragment (aa89-251) (exact sequence is proprietary)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: Aurora-B

Molekulargewicht: 39 kDa

Gen-ID: 9212, 442658

UniProt: Q96GD4

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades