Rekombinanter Melanoma gp100 Antikörper (CF®568)

Produktnummer ABIN6154759

Der Kaninchen Monoklonal Anti-Melanoma gp100-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, Melanoma gp100 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Melanoma gp100 Antikörper (CF®568) (ABIN6154759)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist konjugiert mit CF®568

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: PMEL-1825R

Produktdetails anti-Melanoma gp100 Antikörper (CF®568)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-gp100 / Melanosome / PMEL17 / SILV (PMEL/1825R), CF568 Conjugate

Produktmerkmale: Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human SILV protein

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

SK-MEL-28 cells or Melanoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: gp100 / Melanosome / PMEL17 / SILV

Molekulargewicht: 90-100 kDa

Gen-ID: 6490, 95972

UniProt: P40967