WT1 Antikörper (Biotin)

Produktnummer ABIN6154432

Produktdetails anti-WT1 Antikörper (Biotin)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-WT1 (Wilm's Tumor 1) (WT1/857), Biotin Conjugate

Produktmerkmale: This antibody recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human WT1 protein

Klon: WT1-857

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1 (Wilm's Tumor 1) (WT1 Produkte)

Synonyme: AWT1 antikoerper, EWS-WT1 antikoerper, GUD antikoerper, NPHS4 antikoerper, WAGR antikoerper, WIT-2 antikoerper, WT33 antikoerper, CWT1 antikoerper, WT1 antikoerper, XWT1a antikoerper, wt-1 antikoerper, wt1 antikoerper, xWT1 antikoerper, WT-1 antikoerper, gud antikoerper, wagr antikoerper, wt33 antikoerper, xwt1 antikoerper, wit-2 antikoerper, si:ch211-160j6.3 antikoerper, zgc:152697 antikoerper, D630046I19Rik antikoerper, Wt-1 antikoerper, XWT1b antikoerper, XeWT1 antikoerper, Wilms tumor 1 antikoerper, Wilms tumor 1 S homeolog antikoerper, wilms tumor 1b antikoerper, Wilms tumor protein antikoerper, Wilms tumor 1 homolog antikoerper, Wilms tumor 1 L homeolog antikoerper, WT1 antikoerper, wt1.S antikoerper, wt1 antikoerper, wt1b antikoerper, Wt1 antikoerper, wt1.L antikoerper

Hintergrund: WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1

Molekulargewicht: 47-55 kDa

Gen-ID: 7490, 591980

UniProt: P19544

Pathways: Tube Formation