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HLA-Aw32-&-HLA-A25 (MHC-I) Antikörper (CF®647)

This anti- antibody (ABIN6152739) is a Mouse Monoclonal antibody detecting in FACS, IF, IHC (f). Suitable for Human.
Produktnummer ABIN6152739

Kurzübersicht für HLA-Aw32-&-HLA-A25 (MHC-I) Antikörper (CF®647) (ABIN6152739)

Target

HLA-Aw32-&-HLA-A25 (MHC-I)

Reaktivität

Human

Wirt

  • 8
Maus

Klonalität

  • 8
Monoklonal

Konjugat

  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
CF®647

Applikation

  • 8
  • 8
  • 6
  • 2
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon

CATA-1
  • Verwendungszweck

    Mouse Monoclonal anti-HLA-Aw32-&-HLA-A25 (MHC-I) (CATA-1), CF647 Conjugate

    Produktmerkmale

    This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10

    Isotyp

    IgG2a kappa
  • Applikationshinweise

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    Kommentare

    THP-1 and Jurkat cells. Tonsil or lymph node.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Protect from light
  • Target

    HLA-Aw32-&-HLA-A25 (MHC-I)

    Hintergrund

    HLA-A, HLA class 1A, MHC class I HLA-A

    Molekulargewicht

    kDa

    Gen-ID

    3105, 181244, 654404

    UniProt

    P01889, P30443, P30499
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