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HLA-Aw32-&-HLA-A25 (MHC-I) Antikörper (CF®568)

Reaktivität: Human FACS, IF, IHC (f) Wirt: Maus Monoclonal CATA-1 CF®568
Produktnummer ABIN6152745
  • Target
    HLA-Aw32-&-HLA-A25 (MHC-I)
    Reaktivität
    Human
    Wirt
    • 8
    Maus
    Klonalität
    • 8
    Monoklonal
    Konjugat
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    CF®568
    Applikation
    • 8
    • 8
    • 6
    • 2
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Verwendungszweck
    Mouse Monoclonal anti-HLA-Aw32-&-HLA-A25 (MHC-I) (CATA-1), CF568 Conjugate
    Produktmerkmale
    This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10
    Klon
    CATA-1
    Isotyp
    IgG2a kappa
  • Applikationshinweise
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    THP-1 and Jurkat cells. Tonsil or lymph node.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    HLA-Aw32-&-HLA-A25 (MHC-I)
    Hintergrund
    HLA-A, HLA class 1A, MHC class I HLA-A
    Molekulargewicht
    kDa
    Gen-ID
    3105, 181244, 654404
    UniProt
    P01889, P30443, P30499
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