CD59 Antikörper (CF®647)

Produktnummer ABIN6151675

Der Maus Monoklonal Anti-CD59-Antikörper wurde für FACS und IF validiert. Er ist geeignet, CD59 in Proben von Human zu detektieren.

Kurzübersicht für CD59 Antikörper (CF®647) (ABIN6151675)

Target: CD59

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD59 Antikörper ist konjugiert mit CF®647

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: BRA-10G

Produktdetails anti-CD59 Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-CD59 / Protectin Heavy Chain (BRA-10G), CF647 Conjugate

Produktmerkmale: This antibody reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human K562 tumor cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD59

Target: CD59

Andere Bezeichnung: CD59 (heavy chain of Protectin)

Molekulargewicht: 20 kDa

Gen-ID: 966, 278573

UniProt: P13987

Pathways: Komplementsystem