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CD44 Standard Antikörper (CF®647)

CD44s Reaktivität: Human, Non-Human Primate FACS, IF, IHC (f) Wirt: Maus Monoclonal HCAM-918 CF®647
Produktnummer ABIN6151561
  • Target Alle CD44 Standard (CD44s) Antikörper anzeigen
    CD44 Standard (CD44s)
    Reaktivität
    • 30
    • 12
    • 9
    • 9
    • 8
    • 8
    Human, Non-Human Primate
    Wirt
    • 37
    • 1
    Maus
    Klonalität
    • 38
    Monoklonal
    Konjugat
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD44 Standard Antikörper ist konjugiert mit CF®647
    Applikation
    • 38
    • 30
    • 30
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Verwendungszweck
    Mouse Monoclonal anti-CD44 Standard (HCAM/918), CF647 Conjugate
    Produktmerkmale
    This antibody recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human HCAM protein
    Klon
    HCAM-918
    Isotyp
    IgG2a kappa
    Top Product
    Discover our top product CD44s Primärantikörper
  • Applikationshinweise
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    DU145 cells. Tonsil, lymph node or breast carcinomas.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    CD44 Standard (CD44s)
    Andere Bezeichnung
    CD44 Standard (CD44s Produkte)
    Molekulargewicht
    80-95 kDa
    Gen-ID
    960, 502328
    UniProt
    P16070
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