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CD44 Standard Antikörper

CD44s Reaktivität: Human, Pavian, Green Monkey FACS, IF, IHC (f), WB Wirt: Maus Monoclonal 156-3C11 unconjugated
Produktnummer ABIN6151476
  • Target Alle CD44 Standard (CD44s) Antikörper anzeigen
    CD44 Standard (CD44s)
    Reaktivität
    • 30
    • 12
    • 9
    • 8
    • 8
    • 8
    Human, Pavian, Green Monkey
    Wirt
    • 37
    • 1
    Maus
    Klonalität
    • 38
    Monoklonal
    Konjugat
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD44 Standard Antikörper ist unkonjugiert
    Applikation
    • 38
    • 30
    • 30
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
    Verwendungszweck
    Mouse Monoclonal anti-CD44 Standard (156-3C11), Purified, BSA-free
    Produktmerkmale
    This antibody recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Aufreinigung
    Purified
    Immunogen
    Stimulated human leukocytes
    Klon
    156-3C11
    Isotyp
    IgG2a kappa
    Top Product
    Discover our top product CD44s Primärantikörper
  • Applikationshinweise
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    HeLa cells or paracortex in tonsil or lymph node.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS (no BSA, no azide)
    Konservierungsmittel
    Azide free
  • Target
    CD44 Standard (CD44s)
    Andere Bezeichnung
    CD44 Standard (CD44s Produkte)
    Molekulargewicht
    80-95 kDa
    Gen-ID
    960, 502328
    UniProt
    P16070
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