Vinculin Antikörper

Produktnummer ABIN604849

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Vinculin in WB, IHC, IF, IP und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Vinculin Antikörper (ABIN604849)

Target: Vinculin (VCL)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Vinculin Antikörper

Marke: IHC-plus™

Spezifität: Recognizes human vinculin.

Aufreinigung: Protein G purified

Immunogen: Semi-purified vinculin. MW of Antigen: 130kD (vinculin) and 150kD (meta-vinculin). Cellular Localization: Cell membrane and cytoplasmic.

Type of Immunogen: Protein extract

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-P (2.5 - 5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Studies of vinculin in platelet activation. Immunoprecipitation (Denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2hrs at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives or stabilizing proteins added.

Konservierungsmittel: Azide free

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: VCL / Vinculin

Hintergrund: Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

Gen-ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2