LGALS14 Antikörper (AA 1-139)

Produktnummer ABIN603241

Produktdetails anti-LGALS14 Antikörper

Target: LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))

Bindungsspezifität: AA 1-139

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LGALS14 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.

Aufreinigung: Immunoaffinity purified

Sterilität: Sterile filtered

Immunogen: Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14, aa 1-139, Accession# Q8TCE9).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), WB (2 μg/mL)

Usage: Suitable for use in Western blot: 2 μg/mL with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10 μg total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25 mM Tris,  pH 7.4 5 % nonfat dry milk 5 % nonfat dry milk 0.15 M NaCl in Blotting Buffer in Blotting Buffer 0.05 % Tween 20. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from sterile-filtered PBS, 5 % trehalose

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.

Details zu LGALS14

Target: LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))

Andere Bezeichnung: LGALS14 / CLC2 (LGALS14 Produkte)

Synonyme: CLC2 antikoerper, PPL13 antikoerper, galectin 14 antikoerper, lectin, galactoside-binding, soluble, 14 antikoerper, galectin-14 antikoerper, LGALS14 antikoerper, LOC443162 antikoerper

Hintergrund: Name/Gene ID: LGALS14

Synonyms: LGALS14, CLC2, Gal-14, Galectin-14, Placental protein 13-like, PPL13

Gen-ID: 56891

UniProt: Q8TCE9