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LGALS14 Antikörper (AA 1-139)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch LGALS14 in ELISA und WB. Er zeigt eine Reaktivität gegenüber Human und Maus.
Produktnummer ABIN603241

Kurzübersicht für LGALS14 Antikörper (AA 1-139) (ABIN603241)

Target

LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))

Reaktivität

  • 14
  • 1
  • 1
  • 1
  • 1
Human, Maus

Wirt

  • 15
  • 2
Maus

Klonalität

  • 15
  • 2
Monoklonal

Konjugat

  • 13
  • 2
  • 1
  • 1
Dieser LGALS14 Antikörper ist unkonjugiert

Applikation

  • 13
  • 8
  • 7
  • 6
  • 5
  • 2
ELISA, Western Blotting (WB)
  • Bindungsspezifität

    • 6
    • 3
    • 1
    • 1
    AA 1-139

    Spezifität

    Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.

    Aufreinigung

    Immunoaffinity purified

    Sterilität

    Sterile filtered

    Immunogen

    Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14, aa 1-139, Accession# Q8TCE9).

    Isotyp

    IgG2b
  • Applikationshinweise

    Approved: ELISA (0.5 - 1 μg/mL), WB (2 μg/mL)

    Usage: Suitable for use in Western blot: 2 μg/mL with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10 μg total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25 mM Tris,  pH 7.4 5 % nonfat dry milk 5 % nonfat dry milk 0.15 M NaCl in Blotting Buffer in Blotting Buffer 0.05 % Tween 20. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3 ng/well.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Sterile PBS

    Konzentration

    Lot specific

    Buffer

    Lyophilized from sterile-filtered PBS, 5 % trehalose

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
  • Target

    LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))

    Andere Bezeichnung

    LGALS14 / CLC2

    Hintergrund

    Name/Gene ID: LGALS14

    Synonyms: LGALS14, CLC2, Gal-14, Galectin-14, Placental protein 13-like, PPL13

    Gen-ID

    56891

    UniProt

    Q8TCE9
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