LGALS14 Antikörper (AA 1-139)
Kurzübersicht für LGALS14 Antikörper (AA 1-139) (ABIN603241)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 1-139
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Spezifität
- Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.
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Aufreinigung
- Immunoaffinity purified
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Sterilität
- Sterile filtered
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Immunogen
- Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14, aa 1-139, Accession# Q8TCE9).
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Isotyp
- IgG2b
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Applikationshinweise
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Approved: ELISA (0.5 - 1 μg/mL), WB (2 μg/mL)
Usage: Suitable for use in Western blot: 2 μg/mL with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10 μg total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25 mM Tris, pH 7.4 5 % nonfat dry milk 5 % nonfat dry milk 0.15 M NaCl in Blotting Buffer in Blotting Buffer 0.05 % Tween 20. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3 ng/well. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Sterile PBS
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Konzentration
- Lot specific
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Buffer
- Lyophilized from sterile-filtered PBS, 5 % trehalose
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
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- LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))
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Andere Bezeichnung
- LGALS14 / CLC2
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Hintergrund
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Name/Gene ID: LGALS14
Synonyms: LGALS14, CLC2, Gal-14, Galectin-14, Placental protein 13-like, PPL13 -
Gen-ID
- 56891
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UniProt
- Q8TCE9
Target
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