SPARC Antikörper

Produktnummer ABIN602083

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch SPARC in IHC, ELISA, FACS und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für SPARC Antikörper (ABIN602083)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser SPARC Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SPARC Antikörper

Spezifität: Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.

Aufreinigung: Protein G purified

Immunogen: Recombinant mouse Osteonectin.

Type of Immunogen: Recombinant protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-Fr (25 μg/mL)

Usage: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 μg/mL). Immunohistochemistry (frozen): 25 μg/mL detects SPARC on mouse ovary tissue. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 25 μg/mL and add 10 μLof this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to adding 10 mL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.

Details zu SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Andere Bezeichnung: Osteonectin / SPARC

Hintergrund: Name/Gene ID: SPARC

Synonyms: SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin

Gen-ID: 6678

UniProt: P09486

Pathways: Autophagie