MYOD1 Antikörper (AA 77-215)

Produktnummer ABIN601982

Der Maus Monoklonal Anti-MYOD1-Antikörper wurde für IHC validiert. Er ist geeignet, MYOD1 in Proben von Human zu detektieren.

Kurzübersicht für MYOD1 Antikörper (AA 77-215) (ABIN601982)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Produktdetails anti-MYOD1 Antikörper

Bindungsspezifität: AA 77-215

Spezifität: Recognizes human MyoD.

Aufreinigung: Protein G purified

Sterilität: 0.2 μm filtered

Immunogen: Recombinant human MyoD, aa 77-215 (E. coli). Accession# (P15172).

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: IHC (10 μg/mL)

Usage: Immunohistochemistry:10 μg/mL to detect MyoD in C2C12 mouse muscle myoblast cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with. PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for. 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4 °C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile 40-50 % glycerol, PBS.

Konzentration: Lot specific

Buffer: Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MYOD / MYOD1

Hintergrund: Name/Gene ID: MYOD1

Synonyms: MYOD1, BHLHc1, Myogenic differentiation 1, Myf-3, MYOD, Myogenic factor 3, PUM, MYF3

Gen-ID: 4654

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development