LSD1 Antikörper

Produktnummer ABIN577699

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LSD1 in WB und IP. Er zeigt eine Reaktivität gegenüber Human und Säugetier.

Kurzübersicht für LSD1 Antikörper (ABIN577699)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reaktivität: Human, Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LSD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-LSD1 Antikörper

Marke: AbX™

Produktmerkmale: Reacts with mammalian Lysine Specific Demethylase 1

Aufreinigung: Purified

Immunogen: Full length recombinant Lysine Specific Demethylase 1

Anwendungsinformationen

Applikationshinweise: Western blotting and Immunoprecipitation
Western blotting, 1:50-1:200 AbX™ Anti-LSD1 Rabbit pAb
Detection of LSD1 in nuclear extract of 20 μg of HeLa cells probed with A003-100UL at a 1:200 dilution. MW of LSD1 is 110 kDa.

Kommentare:

Reacts with mammalian Lysine Specific Demethylase 1 Supplied as a PBS solution Applications include Western blotting and Immunoprecipitation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate Buffered Saline at pH 7.2 containing 0.1 % Tween 20 and 0.09 % Kathon preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C

Informationen zur Lagerung: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Details zu LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Andere Bezeichnung: Lysine Specific Demethylase 1 (LSD1)

Hintergrund: There are two known classes of histone demethylases (HDM), differing in their reaction chemistry, coenzyme use and reaction products. Flavin-dependent HDMs act only on mono- and dimethylated lysines and produce hydrogen peroxide, whereas the Jumonji-containing HDMs are iron-dependent enzymes that can act on mono-, di- and trimethylated lysine side chains, and also on methylated Arginine residues. Both classes also produce formaldehyde as a product of the demethylation reaction. LSD1 (Lysine-specific demethylase 1) is a flavin- dependent amine oxidase and was identified as a subunit of different complexes and has been shown to be involved in transcriptional repression of genes through the demethylation of monomethyl Lys4 and dimethyl Lys4 Histone H3. LSD1 is also involved in transcriptional activation through the demethylation of monomethyl Lys9 and dimethyl Lys9 Histone H3. It is also able to demethylate both mono- and dimethyl Lys370 in the regulatory domain of the tumor suppressor p53.

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effekt