PLA2G4A Antikörper (Internal Region)

Produktnummer ABIN571180

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PLA2G4A in WB, IHC, ELISA, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PLA2G4A Antikörper (Internal Region) (ABIN571180)

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PLA2G4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-PLA2G4A Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PLA2G4A

Sequenz: QEKTFRQQRK EHIR

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QEKTFRQQRKEHIR, from the internal region of the protein sequence according to NP_077734.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 80-85 kDa band observed in lysates of cell line A549 (calculated MW of 85.2 kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90 kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Andere Bezeichnung: PLA2G4A

Hintergrund: PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase

Gen-ID: 5321

NCBI Accession: NP_077734

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling