PLA2G4A Antikörper (Internal Region)

Produktnummer ABIN571180

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PLA2G4A in WB, IHC, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PLA2G4A Antikörper (Internal Region) (ABIN571180)

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PLA2G4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-PLA2G4A Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PLA2G4A

Sequenz: QEKTFRQQRK EHIR

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QEKTFRQQRKEHIR, from the internal region of the protein sequence according to NP_077734.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 80-85 kDa band observed in lysates of cell line A549 (calculated MW of 85.2 kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90 kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Andere Bezeichnung: PLA2G4A

Hintergrund: PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase

Gen-ID: 5321

NCBI Accession: NP_077734

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling