SPRY1 Antikörper (C-Term)

Produktnummer ABIN570913

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch SPRY1 in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für SPRY1 Antikörper (C-Term) (ABIN570913)

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SPRY1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-SPRY1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Sprouty

Sequenz: CPSRGQGKPS

Spezifität: NP_005832.1 and NP_955359.1 represent varients of the same protein.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Rec

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SPRY1

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Andere Bezeichnung: SPRY1

Hintergrund: SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)

Gen-ID: 10252

NCBI Accession: NP_005832, NP_955359

Pathways: EGFR Signaling Pathway, EGFR Downregulation