Rekombinanter SOX10 Antikörper (AA 115-269)

Produktnummer ABIN5707896

Der Kaninchen Monoklonal anti-SOX10 Antikörper wird verwendet zum Nachweis von SOX10 in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Rekombinanter SOX10 Antikörper (AA 115-269) (ABIN5707896)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser SOX10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SOX10-2311R

Produktdetails anti-SOX10 Antikörper

Bindungsspezifität: AA 115-269

Verwendungszweck: Recombinant SOX10 Antibody

Aufreinigung: Protein A affinity chromatography

Immunogen: Amino acids 115-269 of the human protein were used as the immunogen for the recombinant SOX10 antibody.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the recombinant SOX10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant SOX10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Andere Bezeichnung: SOX10

Hintergrund: SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

UniProt: P56693

Pathways: Chromatin Binding