SOX10 Antikörper (AA 115-269)

Produktnummer ABIN5707893

Der Maus Monoklonal Anti-SOX10-Antikörper wurde für IHC (p) validiert. Er ist geeignet, SOX10 in Proben von Human und Maus zu detektieren.

Kurzübersicht für SOX10 Antikörper (AA 115-269) (ABIN5707893)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX10 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RSOX10-1074

Produktdetails anti-SOX10 Antikörper

Bindungsspezifität: AA 115-269

Aufreinigung: Purified

Reinheit: Protein G affinity chromatography

Immunogen: Amino acids 115-269 from the human protein were used as the immunogen for the recombinant SOX10 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant SOX10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. WB: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant SOX10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Andere Bezeichnung: SOX10

Hintergrund: This mAb recognizes a protein of ~50 kDa identified as SOX10. This mAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX10 is moderately to strongly expressed in desmoplastic melanomas. SOX10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX10 expression is also observed in mast cells.

Pathways: Chromatin Binding