SHMT1 Antikörper

Produktnummer ABIN5706931

Der Kaninchen Polyklonal Anti-SHMT1-Antikörper wurde für WB validiert. Er ist geeignet, SHMT1 in Proben von Vicia faba, Spinach, Pisum sativum und Arabidopsis thaliana zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für SHMT1 Antikörper (ABIN5706931)

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Reaktivität: Vicia faba, Spinach, Pisum sativum, Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SHMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SHMT1 Antikörper

Spezifität: The antibody is very specific for salmonid inducible form - hsp70.

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including: Glycine max, Medicago truncatula, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera, algae: Chlamydomonas reinhardii

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 53 kDa (Arabidopsis thaliana)

Aufreinigung: affinity purified

Immunogen: Purified SHMT protein from Spinacia oleracea

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1:5 000 with standard ECL (WB)

Kommentare:

this antibody can be used on total leaf extracts and isolated mitochondria

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water.

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-SHMT1 Antikörper (ABIN5706931)

Wei, Sun, Sandoval, Cross, Gordon, Kang, Roje: "Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana." in: Archives of biochemistry and biophysics, Vol. 536, Issue 1, pp. 87-96, (2013) (PubMed).

Details zu SHMT1

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Andere Bezeichnung: SHMT

Hintergrund: Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.

Molekulargewicht: 53 kDa (Arabidopsis thaliana)