Histone 3 Antikörper (pSer10)

Produktnummer ABIN5706877

Hersteller Produkt- Nr.: 600-401-i74

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Histone 3 in WB, IF, IHC, DB, MA und FM. Er zeigt eine Reaktivität gegenüber Human und C. elegans.

Kurzübersicht für Histone 3 Antikörper (pSer10) (ABIN5706877)

Target: Histone 3 (H3)

Reaktivität: Human, C. elegans

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: pSer10

Hersteller Produkt- Nr.: 600-401-i74

Hersteller: Rockland

Verwendungszweck: Histone H3 phospho S10 Antibody

Kreuzreaktivität (Details): This antibody reacts with human Histone H3.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 pS10 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3pS10

Aufreinigung: Anti-Histone H3 [p Ser10] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [p Ser10] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 10 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:2000

Application Note: Anti-Histone H3 [p Ser10] antibody is tested by Western Blot, Immunofluorescence, and Dot Blot. This antibody is useful for Immunocytochemistry and Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:2000

Other: Dot Blot 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.44 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3

Hintergrund: Background: H3 pS10 is typically associated with activation of transcription of genes associated with histone H3. This modification is linked to the initiation of chromatin condensation in G(2). During mitosis, H3pS10 is required for proper chromosome segregation. Formation of the H3pS10 modification seems to be regulated in part by p53, which interacts with histone modifying complexes. In development of the retina, the presence of H3pS10 is linked to the abundance of eye defects. This developmental effect of H3pS10 seems to be related to Psf2 and GPR84. The human Timeless protein (Tim) regulates the global H3pS10 phosphorylation in G2/M phase. VASP concentrations peak during mitosis in HeLa cells at the same time as H3pS10, indicating that co-responsibility for transition of G2/M phases. AURKB promotes the phosphorylation of histone H3 at pS10. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gen-ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3