Histone 3 Antikörper (H3K9me3)

Produktnummer ABIN5706787

Hersteller Produkt- Nr.: 600-401-i71

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Histone 3 in WB, IF, IHC, ChIP, DB und FM. Er zeigt eine Reaktivität gegenüber Human und C. elegans.

Kurzübersicht für Histone 3 Antikörper (H3K9me3) (ABIN5706787)

Target: Histone 3 (H3)

Reaktivität: Human, C. elegans

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K9me3

Hersteller Produkt- Nr.: 600-401-i71

Hersteller: Rockland

Verwendungszweck: Histone H3 K9me3 Antibody

Kreuzreaktivität (Details): This antibody reacts with human Histone H3.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 trimethyl Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9me3

Aufreinigung: Anti-Histone H3 [Trimethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [Trimethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 9 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1000

Application Note: Anti-Histone H3 [Trimethyl Lys9] antibody is tested by Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:5000

IF Microscopy Dilution: 1:1000

Other: Dot Blot 0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.49 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3

Hintergrund: Background: Transcriptional silencing of specific genes and repetitive elements is known to be regulated partially through methylation of histone H3 at lysine 9, a hallmark of constitutive heterochromatin. In placenta, OCT2 expression and its ultimate function as a cation transporter is linked with the abundance of H3K9me3, when this methylation of histone H3 occurs in excess, mRNA for OCT2 decreases. Centromeres also are distinctly affected by the H3K9me3 modification, in that it co-localizes with several centromeric proteins, and affects the DNA methylation, particularly at the periphery of centromeric DNA. Gene expression is silenced when there is coordination between the H3K9me3 and H3K4modifications. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gen-ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3