Histone 3 Antikörper (H3K9ac, H3K14ac)

Produktnummer ABIN5706782

Hersteller Produkt- Nr.: 600-401-i73

Der Kaninchen Polyklonal Anti-Histone 3-Antikörper wurde für WB, IF, IHC, ChIP, DB, MA und FM validiert. Er ist geeignet, Histone 3 in Proben von Human, Maus und C. elegans zu detektieren.

Kurzübersicht für Histone 3 Antikörper (H3K9ac, H3K14ac) (ABIN5706782)

Target: Histone 3 (H3)

Reaktivität: Human, Maus, C. elegans

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K9ac, H3K14ac

Hersteller Produkt- Nr.: 600-401-i73

Hersteller: Rockland

Verwendungszweck: Histone H3 K9ac/K14ac Antibody

Kreuzreaktivität (Details): This antibody reacts with human Histone H3.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 Ac Lys9 / Ac Lys14 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9ac/K14ac

Aufreinigung: Anti-Histone H3 [ac Lys9, ac Lys14] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [ac Lys9, ac Lys14] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysines 9 and 14 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-Histone H3 [ac Lys9, ac Lys14] antibody is tested for Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:200

Other: Dot Blot 1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.63 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3

Hintergrund: Background: Acetylation at K9 and K14 of histone H3 is known to be an epigenetic modification associated with active transcription. Under cellular stress conditions, H3K9ac/K14ac levels increase, probably due to the inactivation of the HDA6 histone deacetylase. Particularly, during infections with herpes simplex virus, the acute phase of the infection is demarcated by increased presence of H3K9ac/K14ac associated with transcriptionally active euchromatin. The virus seems to take advantage of the highly transcribed regions of euchromatin for viral replication, increasing the lytic infection. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gen-ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3