Histone 3 Antikörper (H3K79me3)

Produktnummer ABIN5706779

Hersteller Produkt- Nr.: 600-401-i95

Der Kaninchen Polyklonal Anti-Histone 3-Antikörper wurde für WB, IF, IHC, ChIP, DB, MA und FM validiert. Er ist geeignet, Histone 3 in Proben von Human und C. elegans zu detektieren.

Kurzübersicht für Histone 3 Antikörper (H3K79me3) (ABIN5706779)

Target: Histone 3 (H3)

Reaktivität: Human, C. elegans

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K79me3

Hersteller Produkt- Nr.: 600-401-i95

Hersteller: Rockland

Verwendungszweck: Histone H3 K79me3 Antibody

Kreuzreaktivität (Details): This antibody reacts with human Histone H3.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 trimethyl Lys79 antibody, H3.3B, H3K27Me3, H3K79Me3, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782

Aufreinigung: Anti-Histone H3 [Trimethyl Lys79] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [Trimethyl Lys79] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 79 of human Histone H3.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100-1:1000

Application Note: Anti-Histone H3 [Trimethyl Lys79] antibody is tested in Western Blot, Dot Blot, Chromatin Immunoprecipitation, and Immunofluorescence. This antibody is useful for Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1:100-1:1000

Other: user optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.6 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3

Hintergrund: Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones, and Phospho Specific research.

Gen-ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3