Histone 3 Antikörper (H3K27me3)

Produktnummer ABIN5706760

Hersteller Produkt- Nr.: 600-401-j99

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Histone 3 in WB, IF, ELISA, IP, DB und FM. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Histone 3 Antikörper (H3K27me3) (ABIN5706760)

Target: Histone 3 (H3)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K27me3

Hersteller Produkt- Nr.: 600-401-j99

Hersteller: Rockland

Verwendungszweck: Histone H3 K27me3 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 trimethyl Lys27 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K27me3

Aufreinigung: Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Histone H3 [Trimethyl Lys27] antibody is tested for Dot Blot, IF, and Western Blot. This antibody is useful for ELISA and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1:1000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1: 10,000

IF Microscopy Dilution: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.93 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3

Hintergrund: Background: The nucleosome is comprised of 146 bp of DNA wrapped around a series of histone proteins arranged as an octamer consisting of 2 copies of histone H2A, H2B, H3 and H4. Within the nucleosome core the histone proteins are covalent modified at specific residues predominantly within the N-terminal tail including lysine (acetylation, methylation, SUMOylation, and ubiquitinylation), arginine methylation and citrullination, serine and threonine phosphorylation, as well as proline isomerization. The lysine side chains can carry up to three methyl groups (mono-, di- and tri- methylated forms) and the arginine side chain can be monomethylated or can be dimethylated as the symmetric or asymmetric forms. The modifications show temporal, disease-specific, and other types of cell-specific regulation and there are specific families of enzymes that regulate the methylation, demethylation, acetylation, deacetylation and other modifications. Research has indicated that whereas the histone mark H3K4Me3 (tri-methyl lysine 4 of histone H3) localizes to gene promoter regions (it is associated with transcriptional activation) other modifications at H3K4 such as monomethyl is present predominantly at enhancer sequences. Specific marks have been shown to be associated with the activation (H3K9Me1, H3K27Me1, and H4K20Me1) or repression (H3K9Me2 and Me3, H3K27Me2 and Me3, and H4K20Me2 and Me3) of genes. Monomethylation of H4 at K20, catalyzed by SET8, is essential to genome replication and stability. Multiple DNA breaks are associated with demethylation at this site, resulting in activation of p53 to avoid mitosis and aberrant chromosomal activity. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones, and Phospho Specific research.

UniProt: Q71DI3