Histone 3 Antikörper (H3K18ac)

Produktnummer ABIN5706753

Hersteller Produkt- Nr.: 600-401-i81

Produktdetails anti-Histone 3 Antikörper

Target: Histone 3 (H3)

Bindungsspezifität: H3K18ac

Reaktivität: Human, Maus, C. elegans

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-i81

Hersteller: Rockland

Verwendungszweck: Histone H3 K18ac Antibody

Kreuzreaktivität (Details): This antibody reacts with human Histone H3.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 Ac Lys18 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3K18ac

Aufreinigung: Anti-Histone H3 [ac Lys18] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [ac Lys18] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 18 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Histone H3 [ac Lys18] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:500

Other: Dot Blot 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3 (H3 Produkte)

Hintergrund: Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, the acetylation of lysine 18 on histone 3 (H3 K18ac) is associated with transcriptional activation, DNA replication, and DNA repair. Enzymes known to acetylate K18 included Gcn5, p300, CBP, and TFIIIC90. Acetylation of this amino acid is also known to potentiate other arginine methylation sites on H3. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones.

Gen-ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3