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IL12A Antikörper (AA 23-215)

Der Kaninchen Polyklonal Anti-IL12A-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, IL12A in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN5693302

Kurzübersicht für IL12A Antikörper (AA 23-215) (ABIN5693302)

Target

Alle IL12A Antikörper anzeigen
IL12A (Interleukin 12 alpha (IL12A))

Reaktivität

  • 62
  • 15
  • 7
  • 6
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 59
  • 28
  • 5
  • 2
  • 2
Kaninchen

Klonalität

  • 66
  • 30
Polyklonal

Konjugat

  • 65
  • 13
  • 7
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser IL12A Antikörper ist unkonjugiert

Applikation

  • 78
  • 53
  • 34
  • 31
  • 25
  • 7
  • 7
  • 6
  • 4
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Bindungsspezifität

    • 17
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 23-215

    Verwendungszweck

    Anti-IL12A Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-IL12A Antibody Picoband® (ABIN5693302). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Immunogen

    E. coli-derived rat IL12A recombinant protein (Position: R23-S215).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    ELISA, 0.1-0.5 μg/mL
    1. "Entrez Gene: IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)". 2. Wolf SF, Temple PA, Kobayashi M, Young D, Dicig M, Lowe L, Dzialo R, Fitz L, Ferenz C, Hewick RM (May 1991). "Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells". J Immunol. 146 (9): 3074-81.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • He, Liang, Guo, Huang, Niu, Dong, Wang, Zhang: "Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy." in: Scientific reports, Vol. 6, pp. 24506, (2017) (PubMed).

  • Target

    IL12A (Interleukin 12 alpha (IL12A))

    Andere Bezeichnung

    Il12a

    Hintergrund

    Synonyms: Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, Il12a

    Tissue Specificity: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.

    Background: Interleukin-12 subunit alpha (IL-12 p35) is a protein that in humans is encoded by the IL12A gene. This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

    Molekulargewicht

    35 kDa

    Gen-ID

    84405

    UniProt

    Q9R103

    Pathways

    JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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