ERN1 Antikörper (AA 158-280)

Produktnummer ABIN5693219

Der Kaninchen Polyklonal anti-ERN1 Antikörper (ABIN5693219) detektiert spezifisch ERN1 in WB, ELISA, FACS, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für ERN1 Antikörper (AA 158-280) (ABIN5693219)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-ERN1 Antikörper

Bindungsspezifität: AA 158-280

Verwendungszweck: Anti-IRE1/ERN1 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-IRE1/ERN1 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human IRE1 recombinant protein (Position: R158-L280).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Andere Bezeichnung: ERN1

Hintergrund:

Background: The serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 (IRE1) is an enzyme that in humans is encoded by the ERN1 gene. This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.

Gene Full Name: endoplasmic reticulum to nucleus signaling 1

Molekulargewicht: 170 kDa

Gen-ID: 81

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response