HSPH1 Antikörper (AA 653-858)

Produktnummer ABIN5693036

Der Kaninchen Polyklonal anti-HSPH1 Antikörper (ABIN5693036) detektiert spezifisch HSPH1 in WB, IHC, ELISA, ICC, IF, FACS und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für HSPH1 Antikörper (AA 653-858) (ABIN5693036)

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-HSPH1 Antikörper

Bindungsspezifität: AA 653-858

Verwendungszweck: Anti-Hsp105/HSPH1 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Hsp105/HSPH1 Antibody (ABIN5693036). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Hsp105 recombinant protein (Position: Y653-D858).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu HSPH1

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Andere Bezeichnung: HSPH1

Hintergrund:

Synonyms: Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, HSPH1, HSP105, HSP110, KIAA0201

Tissue Specificity: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Background: HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.

Molekulargewicht: 105 kDa

Gen-ID: 10808

UniProt: Q92598